Co-organized by James Fraser and Nevan Krogan, we are pleased to invite you to the 2017 QBI (Quantitative Biosciences Institute) Symposia on Spring Mutations: how deep sequencing is changing protein science. It will be the first of a series of QBI symposia that will focus on cutting edge technologies that are revolutionizing biology. This meeting, being co-sponsored by QBI and the Convergence Zone at the Gladstone Institutes, will be held March 14-15, 2017, at the UCSF Campus at Mission Bay in San Francisco, California. The event will start at 12:00pm on March 14, 2017 at the Mahley Auditorium and end at 4pm March 15, 2017.
Confirmed Speakers:
Adam Abate, University of California, San Francisco
Sequence function mapping of enzymes and membrane proteins with microfluidics
Jesse Bloom, Fred Hutchinson Cancer Research Center
Statistical methods to assess the adequacy of deep mutational scanning experiments for describing actual natural selection on protein-coding genes
Dan Bolon, University of Massachusetts
Biochemical and evolutionary lessons from viewing protein fitness landscapes through a next-gen lens
Seemay Chou, University of California, San Francisco
The bacterial cell wall: probing an ancient structure with modern tools
Christian Cunningham, University of California, San Francisco
Using Next Generation Sequencing to Engineer Protein-based Therapeutics
Doug Fowler, University of Washington/ Fred Hutchinson Cancer Research Center
Deep mutational scanning to interpret variants in human genomes
Polly Fordyce, Stanford University
Harnessing microfluidics for high-throughput, quantitative enzymology
Catherine Fox, University of Wisconsin-Madison
Using deep sequencing to define the structure and regulation of yeast DNA replication origins at high-resolution
James Fraser, University of California, San Francisco
Selections under different chemical conditions: leveraging graduate students!
Cory Johannessen, Broad Institute
Systematic mapping of mutant kinase function through saturation mutagenesis
Martin Kampman, University of California, San Francisco
CRISPR-based functional genomics to elucidate therapeutic targets and protein-drug interactions
Tanja Kortemme, University of California, San Francisco
Mutational perturbation of a fundamental biological switch
Sri Kosuri, University of California, Los Angeles
Combining large-scale gene synthesis with new functional screens to explore protein function
Debora Marks, Harvard Medical School
Post-evolutionary biology: mutation effects predicted from genomic sequences
Amit Majithia, Broad Institute
Making sense of missense: Prospective functional characterization of disease relevant proteins
Lianet Noda-Garcia, The Weizmann Institute
Systematic mapping of chance and necessity in protein sequence evolution
Kim Reynolds, University of Texas, Southwestern Medical Center
Evolutionary modularity
Phil Romero, University of Wisconsin, Madison
Dissecting protein function with microfluidic-based deep mutational scanning
Dave Savage, University of California, Berkeley
Synthetic Biological Tools for Probing and Perturbing Cellular Physiology
Lea Starita, University of Washington
Multiplex assays for measuring variant effects
Ren Sun, University of California, Los Angeles
Functional analyses of influenza virus genome at single nucleotide resolution: application in rational design of vaccine for broad protection
Jim Wells, University of California, San Francisco
PhaNGS: A first generation Protein seq
TIMING OF SPEAKERS
DAY ONE March 14, 2017
12:30pm Audience Check In
1:00pm Symposium Begins - Thematic Session # 1 FOUNDATIONAL TECHNOLOGY: chair (James Fraser)
1:00pm James Fraser, UCSF - WELCOME and Selections under different chemical conditions: leveraging graduate students!
1:20pm Doug Fowler, University of Washington/ Fred Hutchinson Cancer Research Center
Deep mutational scanning to interpret variants in human genomes
1:40pm Adam Abate, UCSF
Sequence function mapping of enzymes and membrane proteins with microfluidics
2:00pm Sri Kosuri, University of California, Los Angeles Title TBD
2:20pm COFFEE BREAK Room A/B
2:40pm Thematic Session #2 EVOLUTION (chair:Tina Perica)
2:40pm Debora Marks, Harvard University
Post-evolutionary biology: mutation effects predicted from genomic sequences
3:00pm Dan Bolon, University of Massachusetts
Biochemical and evolutionary lessons from viewing protein fitness landscapes through a next-gen lens
3:20pm Kim Reynolds, University of Texas, Southwestern Medical Center
Evolutionary modularity
3:40pm Lianet Noda-Garcia, The Weizmann Institute
Systematic mapping of chance and necessity in protein sequence evolution
4:00pm COFFEE BREAK Room A/B
Thematic Session #3 POTPURRI (chair: Dan Bolon)
4:20pm LIGHTNING TALKS
4:40pm Seemay Chou, University of California, San Francisco
The bacterial cell wall: probing an ancient structure with modern tools
5:00pm Jesse Bloom, Fred Hutchinson Cancer Research Center
Statistical methods to assess the adequacy of deep mutational scanning experiments for describing actual natural selection on proteincoding genes
DAY TWO March 15, 2017
9:00am SYMPOSIUM BEGINS - Thematic Session # 4 ENGINEERING and THERAPEUTICS (chair:Katie Pollard)
9:00am Martin Kampman, University of California, San Francisco
CRISPR-based functional genomics to elucidate therapeutic targets and protein-drug interactions
9:20am Jim Wells, University of California, San Francisco
PhaNGS: A first generation Protein seq
9:40am Christian Cunningham, University of California, San Francisco
Using Next Generation Sequencing to Engineer Protein-based Therapeutics
10:00am Dave Savage, UC Berkeley
Synthetic Biological Tools for Probing and Perturbing Cellular Physiology
10:20am COFFEE BREAK Room C/D
10:40am Thematic Session #5 ENZYMES AND OTHER SYSTEMS (chair: Martin Kampmann)
10:40am Polly Fordyce, Stanford University
Harnessing microfluidics for high-throughput, quantitative enzymology
11:00am Lea Starita, University of Washington
Multiplex assays for measuring variant effects
11:20am Phil Romero, University of Wisconsin-Madison
Dissecting protein function with microfluidicbased deep mutational scanning
11:40am Catherine Fox, University of Wisconsin-Madison
Using deep sequencing to define the structure and regulation of yeast DNA replication origins at high-resolution
12:00pm LUNCH
1:00pm Thematic Session #6 TOWARDS UNIVERSAL LOOKUP TABLES
(chair: James Fraser)
1:00pm Cory Johannessen, Broad Institute
Systematic mapping of mutant kinase function through saturation mutagenesi
1:20pm Tanja Kortemme, University of California, San Francisco
Mutational perturbation of a fundamental biological switch
1:40pm Amit Majithia, Broad Institute
Making sense of missense: Prospective functional characterization of disease relevant proteins
2:00pm Ren Sun, University of California, Los Angeles
Functional analyses of influenza virus genome at single nucleotide resolution: application in rational design of vaccine for broad protection.
2:30pm END CELEBRATION Room C/D
